Product
Protein Isolation
RIPA lysis buffer performs a strong ability to lyse plasma membrane, cytoplasm and nucleus, besides, it is applicable to either adherent or suspension cell. The total protein which harvested by lysis method is applicable to proteomics approach such as Wetstern Blot, protein immunoprecipitation and reporter assay.
Highlights:
- Broad applications: the lysed protein can be directly used in proteomics approach
- Safe and convenience
Order Information:
| Cat. No. | Product Name | Description |
| RP05-100 | RIPA Cell Lysis Buffer (5X) |
100mL solution |
| RP05-500 | RIPA Cell Lysis Buffer (5X) |
500mL solution |
Product Detail:
Figure 1. Comparison of the cell lysis performance with different sample sources.
The total protein from cell lines (HAP1, Hela, Huh7) extracted by freeze and thaw method and lysed by RIPA Cell Lysis Buffer(#RP05-100 and the two competitors). The results indicate that better protein harvest efficiency performed by VisualProtein Cell Lysis Buffer.
Reference:
1. Ngo, Mai-Huong T., et al. "A yes-associated protein (YAP) and insulin-like growth factor 1 receptor (IGF-1R) signaling loop is involved in Sorafenib resistance in hepatocellular carcinoma." Cancers 13.15 (2021): 3812.
2. Hung, Chia-Yu, et al. "AQP4 Attenuated TRAF6/NFκB Activation in Acrylamide-Induced Neurotoxicity." Molecules 27.3 (2022): 1066.
3. Jen, Chia-I., et al. "Sulfated polysaccharides of Laetiporus sulphureus fruiting bodies exhibit anti-breast cancer activity through cell cycle arrest, apoptosis induction, and inhibiting cell migration." Journal of Ethnopharmacology 321 (2024): 117546.