Product
Western Blots & Immunoassays
LumiFlash™ Ultima Chemiluminescent Substrate, HRP is an advanced ECL product in terms of efficacy and sensitivity for chemiluminescent detection of immobilized proteins in Western blotting. LumiFlash™ Ultima Chemiluminescent Substrate, HRP provides high sensitivity and long signal duration in Western blotting application. It needs very short exposure time to the X-ray film or other documentation systems, and can get low background and high signal with clear image. LumiFlash™ Ultima Chemiluminescent Substrate, HRP is the perfect choice for most Western blotting application.
Highlights:
- High signal sensitivity – detection of target protein at low-picogram level on WB
- Antibody saving – diluted lower antibody concentration for WB
- Long duration – provide stable signal for long exposure
- Economy – cost less than other ECL substrate with similar sensitivity level
Order Information:
| Cat. No. | Product Name | Description |
| LF08-500 | LumiFlash™ Ultima Chemiluminescent Substrate, HRP System |
250mL soultion A + 250mL solution B |
Product Detail:
Figure 1. The comparison of WB application for LumiFlash Ultima, VisGlow Plus, and Luminata Crescendo.
K562 cell lysate with 1/2 serial dilution from 50 µg was separated by 12.5% SDS-PAGE and probed by anti-HSP 47. All results were exposed to X-ray film for 3 minutes.
Figure 2. The comparison of LumiFlash Prime, LumiFlash Ultima, LumiFlash Infinity, and competitors.
Cell lysate of Huh7 with 1/3 serial dilution from 6 µg was separated by 12.5% SDS-PAGE. The proteins were transferred to PVDF and blocked with BlockPRO™ Protein-Free Blocking Buffer(#BF01-1L). The blot was probed at 1:10,000 with anti-GAPDH. HRP-conjugated secondary antibody was applied and developed with a chemiluminescent substrate as indicated. All blots were simultaneously exposed for 15 secs by the ChemLux SPX-600R imaging system.
LumiFlash™ Series:
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