Product

Western Blots & Immunoassays

BlockPRO™ 1 Min Protein-Free Blocking Buffer
Complete the blocking step in only 1 minute.

BlockPRO™ 1 Min Protein-Free Blocking Buffer is a fast blocking buffer for Western blotting with non-protein formulation containing 0.05% Tween-20 surfactant. It effectively prevents protein cross-reaction and improves the signal/noise ratio of the detected signals by one-minute blocking. BlockPRO™ 1 Min Protein-Free Blocking Buffer can be applied to immunodetection systems including antibodies and biotin/avidin systems such as ELISA, Western blotting, immunohistochemistry and other immunochemical applications.
產品資訊

Highlights:

 

  • Fast blocking: the enhanced formula shortens the blocking time to 1 minute
  • Protein-free: eliminates cross-reactivity happened in traditional protein-based blocking buffers
  • Antibody diluent: for diluting primary and secondary antibodies and extending the storage time of antibodies

 

 

Order Information:

Cat. No. Product Name Description
BM01-500

BlockPRO™ 1 Min Protein-Free Blocking Buffer 

500mL Solution X 1 

BM10-100 BlockPRO™ 1 Min Protein-Free Blocking Buffer (10X)

100mL Solution X 1

 

 

 

Product Detail:

 

 

 

Figure 1. BlockPRO™ 1 Min Protein-Free Blocking Buffer enhanced the signal intensity and shorten the blocking time to 1 min. 

20 μg of Huh-7 cell lysates were serial diluted and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 min hour at room temperature with BlockPRO™ 1 Min or 1 hour in competitor's product (as indicated), probed with mouse anti-ACC followed by anti-mouse HRP and detected with LumiFlash™ Ultima Chemiluminescent Substrate.

 

 

Selection Guide:

 

 

Reference:

 

Item Description Application Link
1 Hsueh, Cheng-Shun, et al. "Role of nuclear factor-kappa B in feline injection site sarcoma." BMC veterinary research 15.1 (2019)  WB PMID: 31653220
2 Lin, Yuan‐Hau, et al. “The study of isotopic enrichment of water in human plasma and erythrocyte.” The FASEB Journal 34.9 (2020): 13049-13062.  WB PMID: 32779304
3 Su, Kuan-Wei, et al. “Repurposing cabozantinib with therapeutic potential in KIT-driven t (8; 21) acute myeloid leukaemias.” Cancer Gene Therapy (2021)  WB PMID: 33833412
4 Chen, Shih-Heng, et al. "Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons." International Journal of Molecular Sciences 22.22 (2021)  WB PMID: 34830254
5 Shih, Cheng-Hsin, et al. "Investigating the role of signal transducer and activator of transcription 3 in feline injection site sarcoma." BMC Veterinary Research 18.1 (2022) IHC PMID: 35836213
6 Wang, Ya‐Chin, et al. "NPRL2 down‐regulation facilitates the growth of hepatocellular carcinoma via the mTOR pathway and autophagy suppression." Hepatology Communications 6.12 (2022)  WB PMID: 36321403
7 Chang, Hsin-Han, et al. "ONX-0914 Induces Apoptosis and Autophagy with p53 Regulation in Human Glioblastoma Cells." Cancers 14.22 (2022)  WB PMID: 36428804
8 Tang, Ya-Xin, et al. "Lipophilic constituents in Salvia miltiorrhiza Inhibit activation of the hepatic stellate cells by suppressing the JAK1/STAT3 signaling pathway: A network pharmacology study and experimental validation." Frontiers in Pharmacology 13 (2022)  WB PMID: 35517817
9 Liao, Tzu-Chieh, et al. "Granulocytic MDSC with deficient CCR5 alleviates lipogenesis and inflammation in nonalcoholic fatty liver disease." International Journal of Molecular Sciences 23.21 (2022) WB PMID: 36361830
10 Cheng, Chia-Hsiung, et al. "Plasmon-activated water as a therapeutic strategy in Alzheimer’s disease by altering gut microbiota." Aging (Albany NY) 15.9 (2023)  WB PMID: 37166426
11 Hsiao, Wen-Wei, et al. "Antifungal Activity of Cedrol from Cunninghamia lanceolate var. konishii against Phellinus noxius and Its Mechanism." Plants 13.2 (2024): 321.  WB PMID: 38276778
12 Chen, Shih-Heng, et al. "The Transplantation of 3-Dimensional Spheroids of Adipose-Derived Stem Cells Promotes Achilles Tendon Healing in Rabbits by Enhancing the Proliferation of Tenocytes and Suppressing M1 Macrophages." The American Journal of Sports Medicine (2024)  WB PMID: 38193194
13 Chomthong, Kittima, et al. "Label-free simultaneous detection of quinolone antibiotic residues using an origami paper–based electrochemical immunosensor." Sensors and Actuators B: Chemical 410 (2024) Immunosensor LINK
14 Mana, Natjira, et al. "Naturally Derived Terpenoids Targeting the 3Dpol of Foot-and-Mouth Disease Virus: An Integrated In Silico and In Vitro Investigation." Viruses 16.7 (2024)  Immunoperoxidase Monolayer Assay PMID: 39066290
15 SY Changi, et al. "Arsenic-induced disruption of circadian rhythms and glutamine anaplerosis in human urothelial carcinoma." Journal of Trace Elements in Medicine and Biology (2024)  WB PMID: 39137608
16 Hsiao, He-Yun, et al. "Stabilization of AMPK/PFKL/RPIA in the Glycolytic Bodies Transduces IL6/STAT3 Signal in Hepatocarcinogenesis." bioRxiv (2024)  IF LINK
17 Kao, Chien-Chang, et al. "GW4064 inhibits migration and invasion through cathepsin B and MMP2 downregulation in human bladder cancer." Chemico-Biological Interactions 389 (2024): 110869. WB PMID: 38216027