Product

Western Blotting

BlockPRO™ 1 Min Protein-Free Blocking Buffer
Complete the blocking step in only 1 minute.

BlockPRO™ 1 Min Protein-Free Blocking Buffer is a fast blocking buffer for Western blotting with non-protein formulation containing 0.05% Tween-20 surfactant. It effectively prevents protein cross-reaction and improves the signal/noise ratio of the detected signals by one-minute blocking. BlockPRO™ 1 Min Protein-Free Blocking Buffer can be applied to immunodetection systems including antibodies and biotin/avidin systems such as ELISA, Western blotting, immunohistochemistry and other immunochemical applications.
產品資訊

Highlights:

 

  • Fast blocking: the enhanced formula shortens the blocking time to 1 minute
  • Protein-free: eliminates cross-reactivity happened in traditional protein-based blocking buffers
  • Antibody diluent: for diluting primary and secondary antibodies and extending the storage time of antibodies

 

 

Order Information:

Cat. No. Product Name Description
BM01-500

BlockPRO™ 1 Min Protein-Free Blocking Buffer 

500mL Solution X 1 

BM10-100 BlockPRO™ 1 Min Protein-Free Blocking Buffer (10X)

100mL Solution X 1

 

 

 

Product Detail :

 

 

 

Figure 1. BlockPRO™ 1 Min Protein-Free Blocking Buffer enhanced the signal intensity and shorten the blocking time to 1 min. 

20 μg of Huh-7 cell lysates were serial diluted and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 min hour at room temperature with BlockPRO™ 1 Min or 1 hour in competitor's product (as indicated), probed with mouse anti-ACC followed by anti-mouse HRP and detected with LumiFlash™ Ultima Chemiluminescent Substrate.

 

 

Reference:

1. Hsueh, Cheng-Shun, et al. "Role of nuclear factor-kappa B in feline injection site sarcoma." BMC veterinary research 15.1 (2019): 365.

2. Lin, Yuan‐Hau, et al. "The study of isotopic enrichment of water in human plasma and erythrocyte." The FASEB Journal.