Special Characteristic :

Ultra-Fast Protocol – Complete protein visualization within 5 minutes, ready for imaging or excision.

High Sensitivity – Detects as low as 1 ng of protein, matching silver stain and SYPRO Ruby performance.

Protein-Safe Mechanism – Stains the gel matrix, not the protein, ensuring no interference with protein integrity or downstream analysis.

Downstream Compatibility – The stained bands can be directly excised and used for Western blotting, LC-MS/MS, enzyme digestion, and other analytical workflows.

Environmentally Friendly – The staining solution is non-toxic, non-biohazardous, and safe for both users and the environment.

Long-Term Stability – Highly stable formulation with consistent results and low background over extended use.

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Figure 1: Positive staining vs Reverse staining.

In positive staining methods such as Coomassie or silver staining, the protein bands absorb dye, appearing as dark bands on a clear background. The staining reagent interacts directly with proteins, which can sometimes alter protein structure or interfere with downstream analysis.

In contrast, reverse staining (as used in VisPRO™ 5 Minutes Protein Stain Kit) relies on imidazole–zinc ion precipitation within the gel matrix. Here, the gel background becomes opaque white, while protein bands remain clear and sharply defined.

Figure 2: Operation workflow

The VisPRO™ workflow is designed for simplicity and speed. After electrophoresis, gels are immersed directly in the ready-to-use staining solution in a black background container—no fixation, heating, or destaining required. Within five minutes, protein bands or spots become clearly visible at the nanogram sensitivity level.

Figure 3: Comparison of staining methods

VisPRO™ produces sharp, high-contrast bands comparable to silver and SYPRO Ruby staining — but in a fraction of the time and with full compatibility for downstream analysis, making it an ideal choice for fast, safe, and quantitative protein visualization.

Figure 4: SDS-PAGE Gel Stained with VisPRO™ 5 Minutes Protein Stain Kit

The image shows an SDS-PAGE gel stained with the VisPRO™ 5 Minutes Protein Stain Kit, captured using a standard cellphone camera without any image enhancement. Even under non-laboratory imaging conditions, the clear, high-contrast protein bands are easily visible, demonstrating the strong staining performance and uniform background of the VisPRO™ reverse-staining system.

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