Western Blotting

BlockPRO™ Blocking Buffer
Ready-to-Use Blocking Buffer for Protein Detection.

BlockPRO™ Blocking Buffer is based on single purified protein which is suitable for blocking in Western blot, ELISA, immunohistochemistry and other immunochemical application. It can block excess binding site but not cover on the binding protein and therefore increase the signal intensity. Best for med-high abundant proteins or strong antibody affinity; high background with current blocking buffer; stripping and re-probing Western blots. Present better data result than milk and BSA blocking buffer.
Product information



  • High efficiency: increase signal intensity
  • High compatibility: compatible with ELISA, IHC, WB
  • Simple: safe and ready-to-use



Order Information:


Cat. No. Product Name Description
BP01-1L BlockPRO™ Blocking Buffer

500mL Solution X 2



Product Detail:



Figure 1. Signal strength comparison of Casein, BSA, milk, and BlockPRO™ Blocking Buffer.

Loading 30 μg cell lysate (HepaG2) and detect with anti-AMPK (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP 1:10,000. Membrane: Hyond™ P. Detection: Hyperfilm™ ECL. All results were exposed for 30 seconds and capture by X-ray film.





Figure 2. Blocking with skim milk or BlockPRO™ Blocking Buffer and detect by SEM.

30 μg cell lysate (HepaG2) separated by 12.5% SDS-PAGE and blocking by skim milk or BlockPRO™ Blocking Buffer. Membranes detected by SEM. The SEM result showed that BlockPRO™ Blocking Buffer only blocking on excess binding site and reveal the position of antigen and therefore will not reduce the signal intensity.



Selection Guide:



1. Chao-Chi Yeh et al. “Integrated Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Quantitative Proteomic Analysis Identifies Galectin-1 as a Potential Biomarker for Predicting Sorafenib Resistance in Liver Cancer.” Molecular & Cellular Proteomics (2015) 14: 1527–1545 (ELISA)

2. Wei-Chun Huang et al. “Therapeutic Benefits of Induced Pluripotent Stem Cells in Monocrotaline-Induced Pulmonary Arterial Hypertension.” PLoS ONE (2016)11, 2: e0142476. doi:10.1371/journal.pone.0142476 (WB)

3. Chang, Chiung-Fang, et al. "N-Glycosylation of human R-Spondin 1 is required for efficient secretion and stability but not for its heparin binding ability." International journal of molecular sciences 17.6 (2016): 937. (WB)

4. Lecaros, Rumwald Leo G., et al. "Nanoparticle delivered VEGF-A siRNA enhances photodynamic therapy for head and neck cancer treatment." Molecular Therapy 24.1 (2016): 106-116. (WB)

5. Rybina, Olga Y., et al. "Tissue-specific transcription of the neuronal gene Lim3 affects Drosophila melanogaster lifespan and locomotion." Biogerontology 18.5 (2017): 739-757. (IHC, WB)

6. Su, Nai-Wen, et al. "Metronomic cordycepin therapy prolongs survival of oral cancer-bearing mice and inhibits epithelial-mesenchymal transition." Molecules 22.4 (2017): 629. (IHC)

7. Nien-Tsu Hsieh et al. “MED28 and forkhead box M1 (FOXM1) mediate matrix metalloproteinase 2 (MMP2)‐dependent cellular migration in human nonsmall cell lung cancer (NSCLC) cells.” Journal of Cellular Physiology (2018), (IF)

8. Shih, Cheng-Ping, et al. "Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia." Scientific reports 8.1 (2018): 1-11. (IF)

9. Ryabova, Elena, et al. "Swiss cheese, Drosophila ortholog of hereditary spastic paraplegia gene NTE, maintains neuromuscular junction development and microtubule Network." Drosophila melanogaster: Model for Recent Advances in Genetics and Therapeutics (2018): 209. (IHC)

10. Trostnikov, Mikhail V., et al. "Disordered expression of shaggy, the drosophila gene encoding a serine-threonine protein kinase gsk3, affects the lifespan in a transcript-, stage-, and tissue-specific manner." International journal of molecular sciences 20.9 (2019): 2200. (IHC)

11. Su, Nai-Wen, et al. “Cordycepin, isolated from medicinal fungus Cordyceps sinensis, enhances radiosensitivity of oral cancer associated with modulation of DNA damage repair.” Food and chemical toxicology 124 (2019): 400-410. (IHC)

12. Chang, Jui-Chih, et al. “Mitochondrial transplantation regulates antitumour activity, chemoresistance and mitochondrial dynamics in breast cancer.” Journal of Experimental & Clinical Cancer Research 38.1 (2019): 30. (WB)

13. Pai, Man-Hui, et al. "Antecedent Dietary Glutamine Supplementation Benefits Modulation of Liver Pyroptosis in Mice with Polymicrobial Sepsis." Nutrients 12.4 (2020): 1086. (WB)

14. Bunnoy, Anurak, Uthairat Na-Nakorn, and Prapansak Srisapoome. "Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (Clarias macrocephalus): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM+ Cells in Clarias Catfish." Biomolecules 10.4 (2020): 567. (ELISA, WB)

15. Liao, Ai-Ho, et al. "Ultrasound-induced microbubble cavitation via a transcanal or transcranial approach facilitates inner ear drug delivery." JCI insight (2020). (IF)

16. Liao, K. Y., Chen, C. J., Hsieh, S. K., Pan, P. H., & Chen, W. Y. (2020). Interleukin-13 ameliorates postischemic hepatic gluconeogenesis and hyperglycemia in rat model of stroke. Metabolic Brain Disease, 1-10. (WB)

17. Trostnikov, Mikhail V., et al. "Modulated expression of the protein kinase GSK3 in motor and dopaminergic neurons increases female lifespan in Drosophila melanogaster." Frontiers in genetics 11 (2020): 668. (IHC, WB)

18. Jhuo, Cian-Fen, et al. "Teaghrelin Protects SH-SY5Y Cells against MPP+-Induced Neurotoxicity through Activation of AMPK/SIRT1/PGC-1α and ERK1/2 Pathways." Nutrients 12.12 (2020): 3665. (WB)

19. Chen, Ya-Hui, et al. "Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells." Molecular Medicine Reports 23.1 (2020): 1-1. (WB)

20. Chang, Jui-Chih, et al. "Antitumor Actions of Intratumoral Delivery of Membrane-Fused Mitochondria in a Mouse Model of Triple-Negative Breast Cancers." OncoTargets and Therapy 13 (2020): 5241-5255. (WB)

21. Yu, Cheng-Chia, et al. "Targeting lncRNA H19/miR-29b/COL1A1 Axis Impedes Myofibroblast Activities of Precancerous Oral Submucous Fibrosis." International Journal of Molecular Sciences 22.4 (2021): 2216. (WB)

22. Shih, Cheng-Ping, et al. "Inhibition of Cochlear HMGB1 Expression Attenuates Oxidative Stress and Inflammation in an Experimental Murine Model of Noise-Induced Hearing Loss." Cells 10.4 (2021): 810. (IHC)

23. Peng, Kang-Yung, et al. “Pathophysiological and Pharmacological Characteristics of KCNJ5 157-159delITE Somatic Mutation in Aldosterone-Producing Adenomas.” Biomedicines 9.8 (2021): 1026. (WB)

24. Chang, Ai-Mei, et al. "Effects of a Recombinant Gonadotropin-Releasing Hormone Vaccine on Reproductive Function in Adult Male ICR Mice." Vaccines 9.8 (2021): 808. (ELISA)

25. Lin, Yi-Chun, et al. "Ultrasound Microbubbles Enhance the Efficacy of Insulin-Like Growth Factor-1 Therapy for the Treatment of Noise-Induced Hearing Loss." Molecules 26.12 (2021): 3626. (IHC)

26. Liao, Ai-Ho, et al. “Development of thermosensitive poloxamer 407-based microbubble gel with ultrasound mediation for inner ear drug delivery.” Drug Delivery 28.1 (2021): 1256-1271. (IF)

27. Yeh, C.-H.; Chen, J.; Zheng, G.; Huang, L.; Hsu, Y.-C. Novel Pyropheophorbide Phosphatydic Acids Photosensitizer Combined EGFR siRNA Gene Therapy for Head and Neck Cancer Treatment. Pharmaceutics 2021, 13, 1435. (WB)

28. Hung, Chia-Yu, et al. "AQP4 Attenuated TRAF6/NFκB Activation in Acrylamide-Induced Neurotoxicity." Molecules 27.3 (2022): 1066. (WB)

29. Liao, Hung-Wei, et al. "Characteristics of a Novel ATP2B3 K416_F418delinsN Mutation in a Classical Aldosterone-Producing Adenoma." Cancers 13.18 (2021): 4729. (WB)

30. Lin, Yong-Shiou, et al. "IGF-1 as a Potential Therapy for Spinocerebellar Ataxia Type 3." Biomedicines 10.2 (2022): 505. (WB)

31. Golomidov, Ilia M., et al. "Reduction of the α-synuclein expression promotes slowing down early neuropathology development in the Drosophila model of Parkinson’s disease." Journal of Neurogenetics (2022): 1-10.(WB)

32. Tzeng, Wen-Sheng, et al. "Anti-cancer activity and cellular uptake of 7, 3′, 4′-and 7, 8, 4′-trihydroxyisoflavone in HepG2 cells under hypoxic conditions." Journal of Enzyme Inhibition and Medicinal Chemistry 39.1 (2024): 2288806. (WB)

33. Meachasompop, Pakapon, et al. "Development of immersion and oral bivalent nanovaccines for streptococcosis and columnaris disease prevention in fry and fingerling Asian seabass (Lates calcarifer) nursery farms." Vaccines 12.1 (2023): 17. (ELISA)

34. Lin, Li-Chun, et al. "Oxidative stress mediates the inhibitory effects of Manzamine A on uterine leiomyoma cell proliferation and extracellular matrix deposition via SOAT inhibition." Redox Biology 66 (2023): 102861.(WB)

35. Chitchongyingcharoen, Narong, et al. "Application of human platelet lysate in chondrocyte expansion promotes chondrogenic phenotype and slows senescence progression via BMP–TAK1–p38 pathway." Scientific Reports 13.1 (2023): 21106. (WB)

36.Tsai, Meng-Wei, et al. "Alpha-lipoic acid ameliorates influenza A virus caused acute pneumonia though enhancement of anti-viral T cell immunity and suppression of macrophage activation by inhibiting ERK1/2 signaling pathway." Journal of Functional Foods 116 (2024): 106212. (WB)