Product
Western Blots & Immunoassays
BlockPRO™ Blocking Buffer
Ready-to-Use Blocking Buffer for Protein Detection.
BlockPRO™ Blocking Buffer is based on single purified protein which is suitable for blocking in Western blot, ELISA, immunohistochemistry and other immunochemical application. It can block excess binding site but not cover on the binding protein and therefore increase the signal intensity. Best for med-high abundant proteins or strong antibody affinity; high background with current blocking buffer; stripping and re-probing Western blots. Present better data result than milk and BSA blocking buffer.
BlockPRO™ Blocking Buffer is based on single purified protein which is suitable for blocking in Western blot, ELISA, immunohistochemistry and other immunochemical application. It can block excess binding site but not cover on the binding protein and therefore increase the signal intensity. Best for med-high abundant proteins or strong antibody affinity; high background with current blocking buffer; stripping and re-probing Western blots. Present better data result than milk and BSA blocking buffer.
Product information
Highlights:
- High efficiency: increase signal intensity
- High compatibility: compatible with ELISA, IHC, WB
- Simple: safe and ready-to-use
Order Information:
| Cat. No. | Product Name | Description |
| BP01-1L | BlockPRO™ Blocking Buffer |
500mL Solution X 2 |
Product Detail:
Figure 1. Signal strength comparison of Casein, BSA, milk, and BlockPRO™ Blocking Buffer.
Loading 30 μg cell lysate (HepaG2) and detect with anti-AMPK (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP 1:10,000. Membrane: Hyond™ P. Detection: Hyperfilm™ ECL. All results were exposed for 30 seconds and capture by X-ray film.
Figure 2. Blocking with skim milk or BlockPRO™ Blocking Buffer and detect by SEM.
30 μg cell lysate (HepaG2) separated by 12.5% SDS-PAGE and blocking by skim milk or BlockPRO™ Blocking Buffer. Membranes detected by SEM. The SEM result showed that BlockPRO™ Blocking Buffer only blocking on excess binding site and reveal the position of antigen and therefore will not reduce the signal intensity.
Selection Guide:
Reference:
| No. | Description | Application | Link |
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| 2 | Lecaros, Rumwald Leo G., et al. "Nanoparticle delivered VEGF-A siRNA enhances photodynamic therapy for head and neck cancer treatment." Molecular Therapy 24.1 (2016) | WB | PMID: 26373346 |
| 3 | Chang, Chiung-Fang, et al. "N-Glycosylation of human R-Spondin 1 is required for efficient secretion and stability but not for its heparin binding ability." International journal of molecular sciences 17.6 (2016) | WB | PMID: 27314333 |
| 4 | Wei-Chun Huang et al. “Therapeutic Benefits of Induced Pluripotent Stem Cells in Monocrotaline-Induced Pulmonary Arterial Hypertension.” PLoS ONE (2016) | WB | PMID: 26840075 |
| 5 | Rybina, Olga Y., et al. "Tissue-specific transcription of the neuronal gene Lim3 affects Drosophila melanogaster lifespan and locomotion." Biogerontology 18.5 (2017) | IHC, WB | PMID: 28466185 |
| 6 | Su, Nai-Wen, et al. "Metronomic cordycepin therapy prolongs survival of oral cancer-bearing mice and inhibits epithelial-mesenchymal transition." Molecules 22.4 (2017) | IHC | PMID: 28406456 |
| 7 | Nien-Tsu Hsieh et al. “MED28 and forkhead box M1 (FOXM1) mediate matrix metalloproteinase 2 (MMP2)‐dependent cellular migration in human nonsmall cell lung cancer (NSCLC) cells.” Journal of Cellular Physiology (2018) | IF | PMID: 30499104 |
| 8 | Shih, Cheng-Ping, et al. "Comparison of changes in mitochondrial bioenergetics between keratinocytes in human external auditory canal skin and cholesteatomas from normoxia to hypoxia." Scientific reports 8.1 (2018) | IF | PMID: 29317713 |
| 9 | Ryabova, Elena, et al. "Swiss cheese, Drosophila ortholog of hereditary spastic paraplegia gene NTE, maintains neuromuscular junction development and microtubule Network." Drosophila melanogaster: Model for Recent Advances in Genetics and Therapeutics (2018) | IHC | LINK |
| 10 | Trostnikov, Mikhail V., et al. "Disordered expression of shaggy, the drosophila gene encoding a serine-threonine protein kinase gsk3, affects the lifespan in a transcript-, stage-, and tissue-specific manner." International journal of molecular sciences 20.9 (2019) | IHC, WB | PMID: 31060255 |
| 11 | Su, Nai-Wen, et al. "Cordycepin, isolated from medicinal fungus Cordyceps sinensis, enhances radiosensitivity of oral cancer associated with modulation of DNA damage repair." Food and chemical toxicology 124 (2019) | IHC | PMID: 30576710 |
| 12 | Chang, Jui-Chih, et al. "Mitochondrial transplantation regulates antitumour activity, chemoresistance and mitochondrial dynamics in breast cancer." Journal of Experimental & Clinical Cancer Research 38.1 (2019) | WB | PMID: 30674338 |
| 13 | Pai, Man-Hui, et al. "Antecedent Dietary Glutamine Supplementation Benefits Modulation of Liver Pyroptosis in Mice with Polymicrobial Sepsis." Nutrients 12.4 (2020) | WB | PMID: 32295272 |
| 14 | Bunnoy, Anurak, Uthairat Na-Nakorn, and Prapansak Srisapoome. "Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (Clarias macrocephalus): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM+ Cells in Clarias Catfish." Biomolecules 10.4 (2020) | ELISA, WB | PMID: 32272764 |
| 15 | Liao, Ai-Ho, et al. "Ultrasound-induced microbubble cavitation via a transcanal or transcranial approach facilitates inner ear drug delivery." JCI insight (2020) | IF | PMID: 37895697 |
| 16 | Liao KY, Chen CJ, et al. Interleukin-13 ameliorates postischemic hepatic gluconeogenesis and hyperglycemia in rat model of stroke. Metab Brain Dis. (2020) | WB | PMID: 32632665 |
| 17 | Trostnikov, Mikhail V., et al. "Modulated expression of the protein kinase GSK3 in motor and dopaminergic neurons increases female lifespan in Drosophila melanogaster." Frontiers in genetics 11 (2020) | IHC, WB | PMID: 32695143 |
| 18 | Jhuo, Cian-Fen, et al. "Teaghrelin Protects SH-SY5Y Cells against MPP+-Induced Neurotoxicity through Activation of AMPK/SIRT1/PGC-1α and ERK1/2 Pathways." Nutrients 12.12 (2020) | WB | PMID: 33260513 |
| 19 | Chen, Ya-Hui, et al. "Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells." Molecular Medicine Reports 23.1 (2020) | WB | PMID: 33236135 |
| 20 | Chang, Jui-Chih, et al. "Antitumor Actions of Intratumoral Delivery of Membrane-Fused Mitochondria in a Mouse Model of Triple-Negative Breast Cancers." OncoTargets and Therapy 13 (2020) | WB | PMID: 32606744 |
| 21 | Trostnikov MV, Veselkina ER, et al. Modulated Expression of the Protein Kinase GSK3 in Motor and Dopaminergic Neurons Increases Female Lifespan in Drosophila melanogaster. Front Genet. (2020) | WB | PMID: 32695143 |
| 22 | Yu, Cheng-Chia, et al. "Targeting lncRNA H19/miR-29b/COL1A1 Axis Impedes Myofibroblast Activities of Precancerous Oral Submucous Fibrosis." International Journal of Molecular Sciences 22.4 (2021) | WB | PMID: 33672311 |
| 23 | Shih, Cheng-Ping, et al. "Inhibition of Cochlear HMGB1 Expression Attenuates Oxidative Stress and Inflammation in an Experimental Murine Model of Noise-Induced Hearing Loss." Cells 10.4 (2021) | IHC | PMID: 33916471 |
| 24 | Peng, Kang-Yung, et al. “Pathophysiological and Pharmacological Characteristics of KCNJ5 157-159delITE Somatic Mutation in Aldosterone-Producing Adenomas.” Biomedicines 9.8 (2021): 1026. | WB | PMID: 34440230 |
| 25 | Chang, Ai-Mei, et al. "Effects of a Recombinant Gonadotropin-Releasing Hormone Vaccine on Reproductive Function in Adult Male ICR Mice." Vaccines 9.8 (2021): 808. | ELISA | PMID: 34451933 |
| 26 | Lin, Yi-Chun, et al. "Ultrasound Microbubbles Enhance the Efficacy of Insulin-Like Growth Factor-1 Therapy for the Treatment of Noise-Induced Hearing Loss." Molecules 26.12 (2021): 3626. | IHC | PMID: 34199327 |
| 27 | Liao, Ai-Ho, et al. “Development of thermosensitive poloxamer 407-based microbubble gel with ultrasound mediation for inner ear drug delivery.” Drug Delivery 28.1 (2021): 1256-1271. | IF | PMID: 34142922 |
| 28 | Yeh, C.-H.; Chen, J.; Zheng, G.; Huang, L.; Hsu, Y.-C. Novel Pyropheophorbide Phosphatydic Acids Photosensitizer Combined EGFR siRNA Gene Therapy for Head and Neck Cancer Treatment. Pharmaceutics 2021, 13, 1435. | WB | PMID: 34575510 |
| 29 | Liao, Hung-Wei, et al. "Characteristics of a Novel ATP2B3 K416_F418delinsN Mutation in a Classical Aldosterone-Producing Adenoma." Cancers 13.18 (2021) | WB | PMID: 34572956 |
| 30 | Hsu, Chih-Hsin, et al. "miR-29a-3p/THBS2 axis regulates PAH-induced cardiac fibrosis." International journal of molecular sciences 22.19 (2021) | WB | PMID: 34638915 |
| 31 | Chen, Ya-Hui, et al. "Metformin potentiates the anticancer effect of everolimus on cervical cancer in vitro and in vivo." Cancers 13.18 (2021) | WB | PMID: 34572837 |
| 32 | Chen, Tsung-Wei, et al. "Dual inhibition of TGFβ signaling and CSF1/CSF1R reprograms tumor-infiltrating macrophages and improves response to chemotherapy via suppressing PD-L1." Cancer Letters 543 (2022) | WB | PMID: 35718267 |
| 33 | Chang, Jui-Chih, et al. "Regulation of mitochondrial fusion and mitophagy by intra-tumoral delivery of membrane-fused mitochondria or Midiv-1 enhances sensitivity to doxorubicin in triple-negative breast cancer." Biomedicine & Pharmacotherapy 153 (2022) | WB | PMID: 36076583 |
| 34 | Tseng, Chi-Shin, et al. "A Novel Somatic Mutation of CACNA1H p. V1937M in Unilateral Primary Hyperaldosteronism." Frontiers in Endocrinology 13 (2022) | WB | PMID: 35757409 |
| 35 | Hung, Chia-Yu, et al. "AQP4 attenuated TRAF6/NFκB activation in acrylamide-induced neurotoxicity." Molecules 27.3 (2022) | WB | PMID: 35164330 |
| 36 | Bunnoy, Anurak, et al. "Mucoadhesive cationic lipid-based Flavobacterium oreochromis nanoencapsulation enhanced the efficacy of mucoadhesive immersion vaccination against columnaris disease and strengthened immunity in Asian sea bass (Lates calcarifer)." Fish & Shellfish Immunology 127 (2022) | ELISA | PMID: 35779812 |
| 37 | Chiu, Chih-Wei, et al. "Yohimbine, an α2-adrenoceptor antagonist, suppresses PDGF-bb-stimulated vascular smooth muscle cell proliferation by downregulating the plcγ1 signaling pathway." International journal of molecular sciences 23.14 (2022) | WB | PMID: 35887391 |
| 38 | Cheng, Yu-Chieh, et al. "Echinocystic Acid Ameliorates Arthritis in SKG Mice by Suppressing Th17 Cell Differentiation and Human Rheumatoid Arthritis Fibroblast-Like Synoviocytes Inflammation." Journal of Agricultural and Food Chemistry 70.51 (2022) | WB | PMID: 36516328 |
| 39 | Golomidov, Ilia M., et al. "Reduction of the α-synuclein expression promotes slowing down early neuropathology development in the Drosophila model of Parkinson’s disease." Journal of Neurogenetics 36.1 (2022) | WB | PMID: 35467466 |
| 40 | Lai, Cheng-Ta, et al. "Midostaurin modulates tumor microenvironment and enhances efficacy of anti-PD-1 against colon cancer." Cancers 14.19 (2022) | IHC | PMID: 36230769 |
| 41 | Lin, Yong-Shiou, et al. "IGF-1 as a potential therapy for spinocerebellar ataxia type 3." Biomedicines 10.2 (2022) | WB | PMID: 35203722 |
| 42 | Chen, Kuan-Yu, et al. "Equilibrative Nucleoside Transporter 1 is a Target to Modulate Neuroinflammation and Improve Functional Recovery in Mice with Spinal Cord Injury." Molecular Neurobiology 60.1 (2023) | WB | PMID: 36269542 |
| 43 | Bunnoy, Anurak, et al. "Development of a bivalent mucoadhesive nanovaccine to prevent francisellosis and columnaris diseases in Nile tilapia (Oreochromis niloticus)." Fish & Shellfish Immunology 138 (2023) | ELISA | PMID: 37182796 |
| 44 | Teengam, Prinjaporn, et al. "An innovative wireless electrochemical card sensor for field-deployable diagnostics of Hepatitis B surface antigen." Scientific Reports 13.1 (2023) | Immunosensor | PMID: 36864072 |
| 45 | Jhuo, Cian-Fen, et al. "Attenuation of skeletal muscle atrophy induced by dexamethasone in rats by teaghrelin supplementation." Molecules 28.2 (2023) | WB | PMID: 36677745 |
| 46 | Huang KC, Ke TW, et al. Dysfunctional TLR1 reduces the therapeutic efficacy of chemotherapy by attenuating HMGB1-mediated antitumor immunity in locally advanced colorectal cancer. Sci Rep. (2023) | WB | PMID: 37945630 |
| 47 | Lin, Yun-Shan, et al. "Targeting CD73 increases therapeutic response to immunogenic chemotherapy by promoting dendritic cell maturation." Cancer Immunology, Immunotherapy (2023) | WB | PMID: 36881132 |
| 48 | Fergany, Alzahraa, et al. "Deletion of IL-1β exacerbates acrylamide-induced neurotoxicity in mice." Toxicological Sciences (2023) | WB | PMID: 37540211 |
| 49 | Meachasompop, Pakapon, et al. "Development of immersion and oral bivalent nanovaccines for streptococcosis and columnaris disease prevention in fry and fingerling Asian seabass (Lates calcarifer) nursery farms." Vaccines 12.1 (2023): 17. | ELISA | PMID: 38250830 |
| 50 | Huang, Kevin Chih-Yang, et al. "TLR1-N248S polymorphism Reduces the Therapeutic Efficacy of Chemotherapy by Attenuating Antitumor Immunity in Locally Advanced Colorectal Cancer." (2023). | WB | LINK |
| 51 | Lin, Li-Chun, et al. "Oxidative stress mediates the inhibitory effects of Manzamine A on uterine leiomyoma cell proliferation and extracellular matrix deposition via SOAT inhibition." Redox Biology 66 (2023): 102861. | ICC, WB | PMID: 37666118 |
| 52 | Lin, Chih-Yao, et al. "Coating-free culture medium for establishing and maintaining human induced pluripotent stem cells." Cell Transplantation 32 (2023): 09636897231198172. | ICC | PMID: 37698258 |
| 53 | Fergany, Alzahraa Ahmed Mohamed Ibrahim. "Mechanisms of chemical-induced degeneration of monoaminergic neurons." (2023). | WB | LINK |
| 54 | Uchuwittayakul, Anurak, Channarong Rodkhum, and Prapansak Srisapoome. "Production of a monoclonal antibody specific to the IgM heavy chain of Asian seabass (Lates calcarifer Bloch, 1790) and its application in assessing health status following vaccination and challenges with Flavobacterium covae and Streptococcus iniae." Aquaculture (2024): 741445. | ELISA | LINK |
| 55 | Tung, Yu-Tang, et al. "Anti-Inflammatory Effects of Anthocyanin-Enriched Black Soybean Seed Coat (BSSC) Crude Extract on LPS-Induced Acute Liver Injury in Mice." Antioxidants 13.3 (2024): 311. | WB | LINK |
| 56 | Tzeng, Wen-Sheng, et al. "Anti-cancer activity and cellular uptake of 7, 3′, 4′-and 7, 8, 4′-trihydroxyisoflavone in HepG2 cells under hypoxic conditions." Journal of Enzyme Inhibition and Medicinal Chemistry 39.1 (2024): 2288806. | WB | PMID: 38153119 |
| 57 | Jhuo, Cian‐Fen, et al. "Teaghrelin protected dopaminergic neurons in MPTP‐induced Parkinson's disease animal model by promoting PINK1/Parkin‐mediated mitophagy and AMPK/SIRT1/PGC1‐α‐mediated mitochondrial biogenesis." Environmental Toxicology (2024). | WB | PMID: 38153120 |
| 58 | Vanichavetin, Kontee, et al. "Oral booster effects of bivalent nanovaccine-primed fingerlings of Asian seabass (Lates calcarifer, Bloch 1790) to prevent streptococcosis and columnaris diseases." Aquaculture (2024): 741165. | ELISA | LINK |
| 59 | Lin, Yi-Chun, et al. "C-Phycocyanin Attenuates Noise-Induced Cochlear Synaptopathy via the Inhibition of Oxidative Stress and Intercellular Adhesion Molecule-1 in the Cochlea." International Journal of Molecular Sciences 25.10 (2024): 5154. | IF, IHC | PMID: 38791192 |
| 60 | Wu, Wen-Tien, et al. "Lovastatin impairs cellular proliferation and enhances hyaluronic acid production in fibroblast-like synoviocytes." Toxicology in Vitro 97 (2024): 105806. | WB | PMID: 38432573 |
| 61 | Yuan, Stephen Hsien-Chi, et al. "AGR2-mediated unconventional secretion of 14-3-3ε and α-actinin-4, responsive to ER stress and autophagy, drives chemotaxis in canine mammary tumor cells." Cellular & Molecular Biology Letters 29.1 (2024): 84. | WB,IF | PMID: 38822246 |
| 62 | Huang, Yu-Chen, et al. "Nutraceutical Potential of Djulis (Chenopodium formosanum) Hull: Phytochemicals, Antioxidant Activity, and Liver Protection." Antioxidants 13.6 (2024): 721. | WB | LINK |
| 63 | Chen, Yng‐Tay, Tzu‐Jung Lin, and Chia‐Yu Hung. "Blood RNA‐sequencing analysis in acrylamide‐induced neurotoxicity and depressive symptoms in rats." Environmental Toxicology 39.4 (2024): 2316-2325. | WB | PMID: 38152866 |
| 64 | Chung, Yu-Hsuan, et al. "Preclinical Animal Study and Pilot Clinical Trial of Using Enriched Peripheral Blood-Derived Mononuclear Cells for Intervertebral Disc Degeneration." Cell Transplantation 33 (2024) | IHC | PMID: 38173231 |