Western Blotting

BlockPRO™ Blocking Buffer
Ready-to-Use Blocking Buffer for Protein Detection.

BlockPRO™ Blocking Buffer is based on single purified protein which is suitable for blocking in Western blot, ELISA, immunohistochemistry and other immunochemical application. It can block excess binding site but not cover on the binding protein and therefore increase the signal intensity. Best for med-high abundant proteins or strong antibody affinity; high background with current blocking buffer; stripping and re-probing Western blots. Present better data result than milk and BSA blocking buffer.
Product information



  • High efficiency: increase signal intensity
  • High compatibility: compatible with ELISA, IHC, WB
  • Simple: safe and ready-to-use



Order Information:


Cat. No. Product Name Description
BP01-1L BlockPRO™ Blocking Buffer

500mL Solution X 2



Product Detail:



Figure 1. Signal strength comparison of Casein, BSA, milk, and BlockPRO™ Blocking Buffer.

Loading 30 μg cell lysate (HepaG2) and detect with anti-AMPK (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP 1:10,000. Membrane: Hyond™ P. Detection: Hyperfilm™ ECL. All results were exposed for 30 seconds and capture by X-ray film.





Figure 2. Blocking with skim milk or BlockPRO™ Blocking Buffer and detect by SEM.

30 μg cell lysate (HepaG2) separated by 12.5% SDS-PAGE and blocking by skim milk or BlockPRO™ Blocking Buffer. Membranes detected by SEM. The SEM result showed that BlockPRO™ Blocking Buffer only blocking on excess binding site and reveal the position of antigen and therefore will not reduce the signal intensity.




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