Western Blotting

LumiFlash™ Infinity Chemiluminescent Substrate, HRP System
The most sensitive ECL substrate for Western blotting analysis.

LumiFlash™ Infinity Chemiluminescent Substrate, HRP is an enhanced chemiluminescent substrate for high femtogram-level by detection of immobilized proteins (Western blotting). LumiFlash™ Infinity Chemiluminescent Substrate, HRP provides high signal and low background, which allows detection of target protein on PVDF or nitrocellulose. This feature benefits the researchers with excellent low background result and without signal burn effect at the same time.
Product information



  • High sensitivity: for quantitative analysis of protein at high femtogram level
  • Antibody saving: recommend dilution of the primary antibody at 5,000-fold and secondary antibody at 50,000 to 250,000-fold (from 1mg/mL stock)
  • Low background: avoid non-specific signals on the WB result



Order Information:


Cat. No. Product Name Description
LF16-500 LumiFlash™ Infinity Chemiluminescent Substrate, HRP System

 250mL Solution A + 250mL Solution B




Product Detail:




Figure 1. The comparison of WB application for LumiFlash Infinity and Luminata Forte.

Hela cell lysate with 1/2 serial dilution from 20 µg was separated by 12.5% SDS-PAGE and probed by anti AMPKa1. All results were exposed to X-ray film for 5 minutes.


Figure 2.  The comparison of LumiFlash Prime, LumiFlash Ultima, LumiFlash Infinity, and competitors.

Cell lysate of Huh7 with 1/3 serial dilution from 6 µg was separated by 12.5% SDS-PAGE. The proteins were transferred to PVDF and blocked with BlockPRO Protein-Free Blocking Buffer(#BF01-1L). The blot was probed at 1:10,000 with anti-GAPDH. HRP-conjugated secondary antibody was applied and developed with a chemiluminescent substrate as indicated. All blots were simultaneously exposed for 15 secs by the ChemLux SPX-600R imaging system.




LumiFlash™ Series:





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7. PARK, Geun-Young, et al. Epithelial-stromal communication via CXCL1-CXCR2 interaction stimulates growth of ovarian cancer cells through p38 activation. Cellular Oncology, 2020, 1-16.