Western Blotting

LumiFlash™ Ultima Chemiluminescent Substrate, HRP System
High signal sensitivity and long signal duration for chemiluminescent.

LumiFlash™ Ultima Chemiluminescent Substrate, HRP is an advanced ECL product in terms of efficacy and sensitivity for chemiluminescent detection of immobilized proteins in Western blotting. LumiFlash™ Ultima Chemiluminescent Substrate, HRP provides high sensitivity and long signal duration in Western blotting application. It needs very short exposure time to the X-ray film or other documentation systems, and can get low background and high signal with clear image. LumiFlash™ Ultima Chemiluminescent Substrate, HRP is the perfect choice for most Western blotting application.
Product information



  • High signal sensitivity –  detection of target protein at low-picogram level on WB 
  • Antibody saving – diluted lower antibody concentration for WB
  • Long duration – provide stable signal for long exposure
  • Economy – cost less than other ECL substrate with similar sensitivity level 



Order Information:


Cat. No. Product Name Description
LF08-500 LumiFlash™ Ultima Chemiluminescent Substrate, HRP System

  250mL soultion A + 250mL solution B




Product Detail:




Figure 1. The comparison of WB application for LumiFlash Ultima, VisGlow Plus, and Luminata Crescendo.

K562 cell lysate with 1/2 serial dilution from 50 µg was separated by 12.5% SDS-PAGE and probed by anti-HSP 47. All results were exposed to X-ray film for 3 minutes.


Figure 2.  The comparison of LumiFlash Prime, LumiFlash Ultima, LumiFlash Infinity, and competitors.

Cell lysate of Huh7 with 1/3 serial dilution from 6 µg was separated by 12.5% SDS-PAGE. The proteins were transferred to PVDF and blocked with BlockPRO Protein-Free Blocking Buffer(#BF01-1L). The blot was probed at 1:10,000 with anti-GAPDH. HRP-conjugated secondary antibody was applied and developed with a chemiluminescent substrate as indicated. All blots were simultaneously exposed for 15 secs by the ChemLux SPX-600R imaging system.



LumiFlash™ Series:






1. Kim, Yunho, et al. "Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis." Nature communications 7 (2016): 10347.

2. Lim, Chae-Seok, et al. "PKCα-mediated phosphorylation of LSD1 is required for presynaptic plasticity and hippocampal learning and memory." Scientific reports 7.1 (2017): 1-15.

3. Lee, I-Ta, et al. "Protective mechanisms of resveratrol derivatives against TNF-α-induced inflammatory responses in rat mesangial cells." Cytokine 113 (2019): 380-392.

4. Cho, Min Young, et al. "Induction of DEAD Box helicase 5 in early adipogenesis is regulated by Ten-eleven translocation 2." Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2020): 158684.

5. Jeong, Gi Uk, et al. "A recombinant human immunoglobulin with coherent avidity to hepatitis B virus surface antigens of various viral genotypes and clinical mutants." PloS one 15.8 (2020): e0236704.

6. Cheng, Ching-Yi, et al. "1, 4-Disubstituted 1H-1, 2, 3-Triazoles for Renal Diseases: Studies of Viability, Anti-Inflammatory, and Antioxidant Activities." International Journal of Molecular Sciences 21.11 (2020): 3823.

7. Kim, Eunju, et al. "Leucrose, a natural sucrose isomer, suppresses dextran sulfate sodium (DSS)-induced colitis in mice by regulating macrophage polarization via JAK1/STAT6 signaling." Journal of Functional Foods 74 (2020): 104156

8. KIM, Min Kyoon, et al. Effects of Steady Low-Intensity Exercise on High-Fat Diet Stimulated Breast Cancer Progression Via the Alteration of Macrophage Polarization. Integrative Cancer Therapies, 2020, 19: 1534735420949678.

9. Chen, H., M. Ko, and H. Chan. "Effects of hyperglycemia on the TGF-β pathway in trabecular meshwork cells." (2021).

10. Tang, Kuo-Tung, et al. "Kurarinone Attenuates Collagen-Induced Arthritis in Mice by Inhibiting Th1/Th17 Cell Responses and Oxidative Stress." International journal of molecular sciences 22.8 (2021): 4002.

11. Pan, Chun-Hsu, et al. "Pathological Role of Phosphoglycerate Kinase 1 in Balloon Angioplasty-Induced Neointima Formation." International Journal of Molecular Sciences 22.16 (2021): 8822.

12. Kwon, Soon Young, et al. "β2‑adrenergic receptor expression and the effects of norepinephrine and propranolol on various head and neck cancer subtypes." Oncology Letters 22.5 (2021): 1-8.

13. Chang, Kai-Wei, et al. "Atractylodin Suppresses TGF-β-Mediated Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells and Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice." International journal of molecular sciences 22.20 (2021): 11152.

14. Lu, I-Ta, et al. "(−)-Agelasidine A Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Human Hepatocellular Carcinoma." Marine Drugs 20.2 (2022): 109.

15. Cheng, Wen-Chien, et al. "Pendulone induces apoptosis via the ROS-mediated ER-stress pathway in human non-small cell lung cancer cells." Toxicology in Vitro 81 (2022): 105346.

16. Lung, Jrhau, et al. "Lipid droplets in lung cancers are crucial for the cell growth and starvation survival." International Journal of Molecular Sciences 23.20 (2022): 12533.

17. Chung, Min Gi, et al. "Bitter taste receptors protect against skin aging by inhibiting cellular senescence and enhancing wound healing." Nutrition Research and Practice 16.1 (2022): 1.

18. Tsai, Sen-Wei, et al. "Erianin alleviates collagen-induced arthritis in mice by inhibiting Th17 cell differentiation." Open Life Sciences 18.1 (2023): 20220703.