Product

Western Blotting

LumiFlash™ Ultima Chemiluminescent Substrate, HRP System
High signal sensitivity and long signal duration for chemiluminescent.

LumiFlash™ Ultima Chemiluminescent Substrate, HRP is an advanced ECL product in terms of efficacy and sensitivity for chemiluminescent detection of immobilized proteins (Western blotting and ELISA). LumiFlash™ Ultima Chemiluminescent Substrate, HRP provides high sensitivity and long signal duration in Western blotting application. It needs very short exposure time to the X-ray film or other documentation systems, and can get low background and high signal with clear image. LumiFlash™ Ultima Chemiluminescent Substrate, HRP is the perfect choice for most Western blotting application.
Product information

Highlights:

 

  • High signal sensitivity –  detection of target protein at low-picogram level on WB and ELISA application
  • Antibody saving – diluted lower antibody concentration for WB and ELISA application
  • Long duration – provide stable signal for long exposure
  • Economy – cost less than other ECL substrate with similar sensitivity level 

 

 

Order Information:

 

Cat. No. Product Name Description
LF08-500 LumiFlash™ Ultima Chemiluminescent Substrate, HRP System

  250mL soultion A + 250mL solution B

 

 

 

Product Detail:

 

         

 

Figure 1. The comparison of WB application for LumiFlash Ultima, VisGlow Plus, and Luminata Crescendo.

K562 cell lysate with 1/2 serial dilution from 50 µg was separated by 12.5% SDS-PAGE and probed by anti-HSP 47. All results were exposed to X-ray film for 3 minutes.

 

Figure 2.  The comparison of LumiFlash Prime, LumiFlash Ultima, LumiFlash Infinity, and competitors.

Cell lysate of Huh7 with 1/3 serial dilution from 6 µg was separated by 12.5% SDS-PAGE. The proteins were transferred to PVDF and blocked with BlockPRO Protein-Free Blocking Buffer(#BF01-1L). The blot was probed at 1:10,000 with anti-GAPDH. HRP-conjugated secondary antibody was applied and developed with a chemiluminescent substrate as indicated. All blots were simultaneously exposed for 15 secs by the ChemLux SPX-600R imaging system.

 

 

LumiFlash™ Series:

 

 

 

 

Reference:

1. Kim, Yunho, et al. "Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis." Nature communications 7 (2016): 10347.

2. Lim, Chae-Seok, et al. "PKCα-mediated phosphorylation of LSD1 is required for presynaptic plasticity and hippocampal learning and memory." Scientific reports 7.1 (2017): 1-15.

3. Lee, I-Ta, et al. "Protective mechanisms of resveratrol derivatives against TNF-α-induced inflammatory responses in rat mesangial cells." Cytokine 113 (2019): 380-392.

4. Cho, Min Young, et al. "Induction of DEAD Box helicase 5 in early adipogenesis is regulated by Ten-eleven translocation 2." Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2020): 158684.