Product

Western Blotting

LumiFlash™ Prime Chemiluminescent Substrate, HRP System
Economic and stable ECL substrate for Western blot.

LumiFlash™ Prime Chemiluminescent Substrate is a ready-to-use reagent for chemiluminescent detection of immobilized proteins (Western blotting), conjugated with horseradish peroxidase (HRP) directly or indirectly. In the presence of hydrogen peroxide, HRP catalyzes the oxidation of cyclic diacylhydrazides, such as luminol, and light emits. LumiFlash™ Prime Chemiluminescent Substrate provides a convenient way to visualize HRP-based detection. Simply mix and add the solutions onto the membrane. The signal of target protein can be recorded by exposure to X-ray film or compatible image acquisition system.
Product information

Highlights:

 

  • High sensitivity – detect protein target to picogram level
  • Antibody saving – less antibody usage with higher quality result on the WB
  • Low background – provide low background on the WB result
  • Economy – offer excellent quality with lower price

 

 

Order Information:

 

Cat. No. Product Name Description
LF01-500 LumiFlash™ Prime Chemiluminescent Substrate, HRP System

 250mL Solution A + 250mL Solution B

 

 

 

Product Detail:

 

       

      

Figure 1.  The comparison of WB application for LumiFlash Prime, VisGlow, and Luminata Crescendo.

Hela cell lysate with 1/2 serial dilution from 20 µg was separated by 12.5% SDS-PAGE and probed by anti b-actin. All results were exposed to X-ray film for 1 minute.

 

Figure 2.  The comparison of LumiFlash Prime, LumiFlash Ultima, LumiFlash Infinity, and competitors.

Cell lysate of Huh7 with 1/3 serial dilution from 6 µg was separated by 12.5% SDS-PAGE. The proteins were transferred to PVDF and blocked with BlockPRO Protein-Free Blocking Buffer(#BF01-1L). The blot was probed at 1:10,000 with anti-GAPDH. HRP-conjugated secondary antibody was applied and developed with a chemiluminescent substrate as indicated. All blots were simultaneously exposed for 15 secs by the ChemLux SPX-600R imaging system.

 

 

 

 

 

LumiFlash™ Series:

 

 

 

 

Reference:

1. Chen, Chin-Chen, et al. "Amelioration of LPS-induced inflammation response in microglia by AMPK activation." BioMed research international 2014 (2014).

2. Young, Guang-Huar, et al. "Identification of adenine modulating AMPK activation in NIH/3T3 cells by proteomic approach." Journal of proteomics 120 (2015): 204-214.

3. Hidalgo-Ruiz, Mario, et al. "Babesia bovis RON2 contains conserved B-cell epitopes that induce an invasion-blocking humoral immune response in immunized cattle." Parasites & vectors 11.1 (2018): 575.

4. RUIZ, MARIO HIDALGO. "Identificación y caracterización inmunológica de epítopos de proteínas involucradas en la invasión de B. bovis a los eritrocitos." (2019).

5. Young, Guang-Huar, et al. "The functional role of hemojuvelin in acute ischemic stroke." Journal of Cerebral Blood Flow & Metabolism (2019): 0271678X19861448

6. Young, Guang-Huar, et al. "Modulation of adenine phosphoribosyltransferase-mediated salvage to promote diabetic wound healing." bioRxiv (2020).