Product

Western Blotting

LuminolPen™, HRP system
Find your disappear molecular weight.

LuminolPen™, HRP System is a perfect tool for chemiluminescent detection in Western blot. With simply writing on the pre-stained marker, the position of the molecular weight can be showed on the image. It could also help you to note the experiment condition or evaluate the efficacy of ECL substrate.
Product information

Highlights:

 

  • Unique: the world's first luminescent color development marker pen
  • Help you to evaluate the efficacy of your ECL substrate
  • Convenient: able to make notes and show on the image with the data result
  • Compatible: applicable with most brands of ECL substrate

 

 

Order Information:

 

Cat. No. Product Name Description
LH03-50 LuminolPen™, HRP System

About 1,000 membranes drawing

LH03-10  LuminolPen™, HRP System

About 100 membranes drawing

 

 

 

Product Detail :

 

 

Figure 1. Labeling molecular weight and note on the membrane with LuminolPen™.

 (A) and (C) are general Western blot chemiluminescence detection image; (B) and (D) are the image using LuminolPen™ to indicate the pre-stained marker.

 

 

 

 

Figure 2. Distinguish target signal and non-specific binding signal with LuminolPen™

30 μg cell lysate SP-1 and detect with anti-ADNP (mouse, 1:2,000). Secondary antibody : anti-mouse IgG-HRP (1:15,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The molecular weight note by LuminolPen™ reveal the correct target signal (ADNP 124KDa) on X-ray film.

 

 

 

 

Figure 3. Evaluate ECL substrate efficacy by LuminolPen™.

30 μg cell lysate HepaG2 and detect with anti-AMP-active protein kinase (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP (1:10,000). Membrane: Hyond™ P. Detection: Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The signal result present by the normal ECL substrate is stronger than using the expired ECL substrate. LuminolPen™ noted marker can be an indicator to check the result or adjust different experiments to similar experimental conditions.

 

 

 

 

 

 

 

Reference:

1. Tsai, Yen-Yin, et al. "Identification of the nanogold particle-induced endoplasmic reticulum stress by omic techniques and systems biology analysis." ACS nano 5.12 (2011): 9354-9369.

2. Chen, Han-Min, Ching-Yu Lin, and Vinchi Wang. "Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach." Clinical biochemistry 44.5 (2011): 377-385.

3. Chiu, Cheng‐Di, et al. "Investigation of the effect of hyperglycemia on intracerebral hemorrhage by proteomic approaches." Proteomics 12.1 (2012): 113-123.

4. Pei-Jung Katy Chung, et al. “microRNA-205 targets tight junction-related proteins during urothelial cellular differentiation.” Molecular & Cellular Proteomics (2014) 13, 9: 2321-2336