Western Blotting

BlockPRO™ 1 Min Protein-Free Blocking Buffer
Complete the blocking step in only 1 minute.

BlockPRO™ 1 Min Protein-Free Blocking Buffer is a fast blocking buffer for Western blotting with non-protein formulation containing 0.05% Tween-20 surfactant. It effectively prevents protein cross-reaction and improves the signal/noise ratio of the detected signals by one-minute blocking. BlockPRO™ 1 Min Protein-Free Blocking Buffer can be applied to immunodetection systems including antibodies and biotin/avidin systems such as ELISA, Western blotting, immunohistochemistry and other immunochemical applications.
Product information



  • Fast blocking: the enhanced formula shortens the blocking time to 1 minute
  • Protein-free: eliminates cross-reactivity happened in traditional protein-based blocking buffers
  • Antibody diluent: for diluting primary and secondary antibodies and extending the storage time of antibodies



Order Information:

Cat. No. Product Name Description

BlockPRO™ 1 Min Protein-Free Blocking Buffer 

500mL Solution X 1 

BM10-100 BlockPRO™ 1 Min Protein-Free Blocking Buffer (10X)

100mL Solution X 1




Product Detail:




Figure 1. BlockPRO™ 1 Min Protein-Free Blocking Buffer enhanced the signal intensity and shorten the blocking time to 1 min. 

20 μg of Huh-7 cell lysates were serial diluted and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 min hour at room temperature with BlockPRO™ 1 Min or 1 hour in competitor's product (as indicated), probed with mouse anti-ACC followed by anti-mouse HRP and detected with LumiFlash™ Ultima Chemiluminescent Substrate.



Selection Guide:




1. Hsueh, Cheng-Shun, et al. "Role of nuclear factor-kappa B in feline injection site sarcoma." BMC veterinary research 15.1 (2019): 365. (WB) Link

2. Lin, Yuan‐Hau, et al. “The study of isotopic enrichment of water in human plasma and erythrocyte.” The FASEB Journal 34.9 (2020): 13049-13062. (WB) Link

3. Su, Kuan-Wei, et al. “Repurposing cabozantinib with therapeutic potential in KIT-driven t (8; 21) acute myeloid leukaemias.” Cancer Gene Therapy (2021): 1-14. (WB) Link

4. Chen, Shih-Heng, et al. "Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons." International Journal of Molecular Sciences 22.22 (2021): 12373. (WB) Link