Product
Western Blotting
BlockPRO™ 1 Min Protein-Free Blocking Buffer is a fast blocking buffer for Western blotting with non-protein formulation containing 0.05% Tween-20 surfactant. It effectively prevents protein cross-reaction and improves the signal/noise ratio of the detected signals by one-minute blocking. BlockPRO™ 1 Min Protein-Free Blocking Buffer can be applied to immunodetection systems including antibodies and biotin/avidin systems such as ELISA, Western blotting, immunohistochemistry and other immunochemical applications.
Highlights:
- Fast blocking: the enhanced formula shortens the blocking time to 1 minute
- Protein-free: eliminates cross-reactivity happened in traditional protein-based blocking buffers
- Antibody diluent: for diluting primary and secondary antibodies and extending the storage time of antibodies
Order Information:
| Cat. No. | Product Name | Description |
| BM01-500 |
BlockPRO™ 1 Min Protein-Free Blocking Buffer |
500mL Solution X 1 |
| BM10-100 | BlockPRO™ 1 Min Protein-Free Blocking Buffer (10X) |
100mL Solution X 1 |
Product Detail:
Figure 1. BlockPRO™ 1 Min Protein-Free Blocking Buffer enhanced the signal intensity and shorten the blocking time to 1 min.
20 μg of Huh-7 cell lysates were serial diluted and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 min hour at room temperature with BlockPRO™ 1 Min or 1 hour in competitor's product (as indicated), probed with mouse anti-ACC followed by anti-mouse HRP and detected with LumiFlash™ Ultima Chemiluminescent Substrate.
Selection Guide:
Reference:
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2. Lin, Yuan‐Hau, et al. “The study of isotopic enrichment of water in human plasma and erythrocyte.” The FASEB Journal 34.9 (2020): 13049-13062. (WB) Link
3. Su, Kuan-Wei, et al. “Repurposing cabozantinib with therapeutic potential in KIT-driven t (8; 21) acute myeloid leukaemias.” Cancer Gene Therapy (2021): 1-14. (WB) Link
4. Chen, Shih-Heng, et al. "Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons." International Journal of Molecular Sciences 22.22 (2021): 12373. (WB) Link