Product
Western Blotting
BlockPRO™ Protein Free Blocking Buffer is a non-protein formulation which enhances sensitivity and minimizes background noise, presenting better results than traditional protein-based blocking buffer in immunoassays. The synthetic formulation of BlockPRO™ Protein-Free Blocking Buffer makes it suitable for PVDF and nitrocellulose platform, avidin/biotin system, detection of phosphoprotein, and other immunochemical applications.
Highlights:
- High performance: Provide better specific signal and less background noise than traditional blocking buffer
- Multiple application: Suitable for Western blot, dot blot, ELISA, and other immunoassays
- High quality control: Ensure lot-to-lot consistency for your most reproducible results over time
Order Information:
| Cat. No. | Product Name | Description |
| BF01-1L | BlockPRO™ Protein-Free Blocking Buffer |
500mL Solution X 2 |
| BF10-100 | BlockPRO™ Protein-Free Blocking Buffer (10X) |
100mL 10X Solution X 1 |
| BF10-200 | BlockPRO™ Protein-Free Blocking Buffer (10X) |
100mL 10X Solution X 2 |
Product Detail:
Figure 1. BlockPRO™ Protein-Free Blocking Buffer is better than protein-based blocking buffers (skim milk, BSA and casein) for detection of target protein in Western blotting.
THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-pAMPK followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.
Figure 2. BlockPRO™ Protein-Free is suitable for various protein antigen detection, such as high molecular weight protein, tACC; phosphoprotein, pAMPK; abundant protein, GAPDH; low molecular weight protein, histone H3.
THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with BlockPRO™ Protein-Free Blocking Buffer. Antibodies designed to probe the indicated proteins were used. All the signals were detected by chemiluminescence and were exposed to X-ray film.
Figure 3. BlockPRO™ Protein-Free Blocking Buffer can be used in both PVDF and nitrocellulose platform.
Hela cell lysates were prepared and separated by SDS-PAGE. The proteins were transferred to PVDF or nitrocellulose membranes. The membranes were blocked for overnight at 4 °C with BlockPRO™ Protein-Free Blocking Buffer or 5% skim milk, probed with mouse anti-histone H3 followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.
Reference:
1. Scheidler, Christopher M., Milan Vrabel, and Sabine Schneider. "Genetic Code Expansion, Protein Expression, and Protein Functionalization in Bacillus subtilis." ACS Synthetic Biology 9.3 (2020): 486-493.
2. CHEN, Kung‑Yen, et al. Monascin accelerates anoikis in circulating tumor cells and prevents breast cancer metastasis. Oncology Letters, 2020, 20.5: 1-1.