BlockPRO™ Protein-Free Blocking Buffer

 

Protein-Free Blocking Buffer for Your Every Need

BlockPRO™ Protein Free Blocking Buffer is a non-protein formulation which enhances sensitivity and minimizes background noise, presenting better results than traditional protein-based blocking buffer in immunoassays. The synthetic formulation of BlockPRO™ Protein-Free Blocking Buffer makes it suitable for PVDF and nitrocellulose platform, avidin/biotin system, detection of phosphoprotein, and other immunochemical applications.

 

 

   Special Characteristic

  • High performance: Provide better specific signal and less background noise than traditional blocking buffer
  • Multiple application: Suitable for Western blot, dot blot, ELISA, and other immunoassays
  • High quality control: Ensure lot-to-lot consistency for your most reproducible results over time

 

  Order Information

Cat. No. Product Name Description

 

BF01-1L

 

  BlockPRO Protein-Free Blocking Buffer

 

  1kit - 500mL Solution X 2

 

 

BF10-100

 

 

  BlockPRO Protein-Free Blocking Buffer (10X)

 

  1kit - 100mL 10X Solution X 1

 

BF10-200

 

 

  BlockPRO Protein-Free Blocking Buffer (10X)

 

  1kit - 100mL 10X Solution X 2

 

 

 

 

  

 

 

 

Product Detail

 

Figure 1. BlockPRO™ Protein-Free Blocking Buffer is better than protein-based blocking buffers (skim milk, BSA and casein) for detection of target protein in Western blotting. THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-pAMPK followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.

 

 

 

Figure 2. BlockPRO™ Protein-Free is suitable for various protein antigen detection, such as high molecular weight protein, tACC; phosphoprotein, pAMPK; abundant protein, GAPDH; low molecular weight protein, histone H3. THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with BlockPRO™ Protein-Free Blocking Buffer. Antibodies designed to probe the indicated proteins were used. All the signals were detected by chemiluminescence and were exposed to X-ray film.

 

 

 

Figure 3. BlockPRO™ Protein-Free Blocking Buffer can be used in both PVDF and nitrocellulose platform.  Hela cell lysates were prepared and separated by SDS-PAGE. The proteins were transferred to PVDF or nitrocellulose membranes. The membranes were blocked for overnight at 4 °C with BlockPRO™ Protein-Free Blocking Buffer or 5% skim milk, probed with mouse anti-histone H3 followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.

 

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