PhosPRO™ Phosphoprotein Purification Kit

New Version of IMAC Based Purification Reagent

PhosPRO™ provides a new version of IMAC based purification method and delivers excellent purification result. PhosPRO™ can specifically purify the phosphoprotein from the protein sample by simple process with our proprietary chromatographic technology. PhosPRO™ can also successfully purify phosphoprotein from different biological materials, such as animal, plant, and microorganism, and is suitable for applying quality required analysis.

 

   Special Characteristic

  • More flexible:PhosPRO™ provides stringent buffer stock for customer to optimize washing condition for their sample and to maximize target phosphoprotein purification result.
     
  1. More choices:PhosPRO™ series have 2 versions with 2 different packages for different purposes

    a. Pre-packed column package is designed for proteomics research and can purify phosphoprotein within 1 hour. The purified sample can directly be analyzed by two-dimensional electrophoresis or mass spectrometry.

    b. Evaluation package is designed for phosphoproteomics research and researcher can small scale optimizes suitable chromatographic conditions for the target phosphoprotein.

 

  Order Information

Cat. No. Product Name Description

 

 

 

 

PP05-2C

 

 

 

 

  PhosPRO™ Phosphoprotein Purification Kit

  1 kit.

  Pre-packed column package

  2 pre-packed column +
  2ml Lysis buffer +
  250ml System buffer +
  100ml Elution buffer +
  100ml stringent buffer stock

 

 

 

 

 

 

PP05-6C

 

 

 

 

 

  PhosPRO™ Phosphoprotein Purification Kit

  1 kit.

  Pre-packed column package

  6 pre-packed column +
  2ml Lysis buffer +
  250ml System buffer +
  100ml Elution buffer +
  100ml stringent buffer stock

 

 

 

 

 

PP05-5E

 

 

 

 

  PhosPRO™ Phosphoprotein Purification Kit

  1 kit.

  Evaluation package 3ml resin +

  2ml Lysis buffer +

  250ml System buffer +

  100ml Elution buffer +

  100ml stringent buffer stock

 

 

 

 

 

 

  Product Detail

 

Figure 1. Purification efficacy of PhosPRO™ Phosphoprotein Purification Kit (A) and Brand Q kit (B). Protein standard markers contain glycogen phosphorylase b (GP), bovine serum albumin (BSA), ovalbumin (OVA), carbonic anhydrase (CA), trypsin inhibitor (TI), and lactalbumin (LAC) were used for evaluation. Ovalbumin was the only phosphoprotein in the test material. F: flow through; E: eluent; VisPRO™ 5 minute Protein Stain Kit (VisPRO™) and Pro-Q Diamond stain (Pro-Q, from Invitrogen) were used for developing total proteins or phosphoproteins.

 

 

Figure 2. Optimize suitable chromatographic condition with PhosPRO™ Phosphoprotein Purification Kit.

 

  Download

MSDS

Manual